PEMBENTUKAN KALUS TANAMAN ROSELLA (Hibiscus sabdariffa) PADA PEMBERIAN NAFTALEN ACETYL ACID (NAA) DAN BENZYL AMINO PURIN (BAP) SEBAGAI SUMBER BELAJAR KONSEP BIOTEKNOLOGI

Sari

Has conducted research for a hand out submateri biotechnology high school students based
on plant tissue culture research Roselle (Hibiscus sabdariffa). The study includes two phases,
namely: (1) Experiments to produce callus tissue culture experiments using completely
randomized design (CRD) factorial. The first factor is the level of NAA treatment that is 0, 1,
1.5, 2 and 3 mg/l. The second factor is the level of BAP treatment with 0, 0.5, 1, and 1.5
mg/l. Each treatment was repeated 3 times; (2) Preparation of handouts from the research as a
source of learning the ADDIE model (Analysis, Design, Development, Implementation, and
Evaluation).Parameters measured were the percentage of growing explants, while appearing
callus and callus texture. Analysis of data growing percentage of explants with advanced
ANOVA and DMRT test at 5% level, while the current and emerging callus texture
descriptively. In a growing percentage of explants parameter combination treatment showed a
percentage of 100 % are in treatment A 0 B 1,5 , A 1 B 0,5 - A 3 B 1,5 . For the current parameters
appear callus treatment best combination found in treatment A 3 B 1,5 with a mean time of 2
HSK appear callus (days after culture) and for the texture parameters of combination
treatment showed callus crumb texture and white color found in treatment A 2 B 0,5 - A 3 B 1,5 , the
research on callus growth can be used as a learning resource in the form of handouts for high
school level students

Keywords : Handout, Tanaman Rosella, Kultur kalus, Hormon NAA dan BAP